Ziv Shah (VI), Sasha Bauhs (VI), Nataly Ruiz (VI), Isabelle Chen (VI), Stella Reheman (V), Katharine Jung (V), James Draper (V), Ishaan Sinha (IV)

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Project Summary

Our group's project involves the expression and characterization of novel fluorescent and luminescent proteins derived from marine organisms. In previous years, we successfully expressed scorpionfish and lizardfish proteins. This year, we are investigating our new protein, UnaG. Currently, we are using various techniques, including gel electrophoresis and bacterial transformation to express and purify our protein, and to induce fluorescence by binding Bilirubin to UnaG. Our ultimate goal is to not only express fluorescence in UnaG, but also express different colors through the use of mutations. We hope to further understand the use of UnaG as a fluorescent marker of gene expression and imaging in living cells. 

Project Goals

• Express and purify our protein
• Induce fluorescence in UnaG
• Change color of UnaG

Faculty Advisor

Dr. Fried

Works Cited:

Kumagai, A., Ando, R., Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., & Miyawaki, A. (n.d.). A bilirubin-inducible fluorescent
     protein from eel muscle. National Library of Medicine. https://pubmed.ncbi.nlm.nih.gov/23768684/